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human serum complement quidel cat  (Quidel)


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    Structured Review

    Quidel human serum complement quidel cat
    Human Serum Complement Quidel Cat, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+complement/Normal+Human+Serum+-+Complement/us12570746-557-1-4
    Average 95 stars, based on 234 article reviews
    human serum complement quidel cat - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Alemtuzumab in Multiple Sclerosis: Short- and Long-Term Effects of Immunodepletion on the Peripheral Treg Compartment
    Article Snippet: .. To screen for possible differences in alemtuzumab effects on Tcon and on Treg subsets, 2 × 10 5 freshly isolated total Treg or Tcon obtained from five healthy subjects were incubated for 3 h at 37° with 10 μg/ml alemtuzumab (Genzyme) or control human IgG (Jackson ImmunoResearch) and 10% normal human complement (Quidel) in X-vivo 15 medium (BioWhittaker) supplemented with 10% FBS (Biochrom). ..

    Incubation:

    Article Title: Alemtuzumab in Multiple Sclerosis: Short- and Long-Term Effects of Immunodepletion on the Peripheral Treg Compartment
    Article Snippet: .. To screen for possible differences in alemtuzumab effects on Tcon and on Treg subsets, 2 × 10 5 freshly isolated total Treg or Tcon obtained from five healthy subjects were incubated for 3 h at 37° with 10 μg/ml alemtuzumab (Genzyme) or control human IgG (Jackson ImmunoResearch) and 10% normal human complement (Quidel) in X-vivo 15 medium (BioWhittaker) supplemented with 10% FBS (Biochrom). ..

    Article Title: IgM anti-ACE2 autoantibodies in severe COVID-19 activate complement and perturb vascular endothelial function
    Article Snippet: To replicate the inflammatory state, microvessels were perfused with EC medium supplemented with 1000 U/mL recombinant human IFN-α2 (BioLegend) and 50 ng/mL recombinant human IFN-γ (R&D Systems) for 24 hours. .. Seventy-two hours after initial cell seeding (24 hours after incubation with IFNs), microvessels were treated with 100 μg/mL patient IgM in EC medium supplemented with 10% normal human complement (Quidel) for 30 minutes under low-flow conditions (~0.3 dyne/cm 2 ). ..

    Article Title: Impact of alemtuzumab treatment on the survival and function of human regulatory T cells in vitro
    Article Snippet: .. Complement-dependent cytolysis assay Enriched T cells were incubated for 1 hr at 37° with 10 μg/ml alemtuzumab or control human IgG (Jackson ImmunoResearch, West Grove, PA) and 10% (volume/volume) normal human complement (Quidel, San Diego, CA) at 2·5 x 10 6 cells/ml in complete AIM-V medium (AIM-V supplemented with 10% fetal bovine serum, 100 units/ml penicillin-streptomycin, 2 mM glutamine and 50 μ m β-mercaptoethanol; Life Technologies, Grand Island, NY). ..

    Article Title: Impact of alemtuzumab treatment on the survival and function of human regulatory T cells in vitro
    Article Snippet: .. Enriched T cells were incubated for 1 hr at 37° with 10 μg/ml alemtuzumab or control human IgG (Jackson ImmunoResearch, West Grove, PA) and 10% (volume/volume) normal human complement (Quidel, San Diego, CA) at 2·5 x 10 6 cells/ml in complete AIM-V medium (AIM-V supplemented with 10% fetal bovine serum, 100 units/ml penicillin-streptomycin, 2 mM glutamine and 50 μ m β-mercaptoethanol; Life Technologies, Grand Island, NY). ..

    Control:

    Article Title: Alemtuzumab in Multiple Sclerosis: Short- and Long-Term Effects of Immunodepletion on the Peripheral Treg Compartment
    Article Snippet: .. To screen for possible differences in alemtuzumab effects on Tcon and on Treg subsets, 2 × 10 5 freshly isolated total Treg or Tcon obtained from five healthy subjects were incubated for 3 h at 37° with 10 μg/ml alemtuzumab (Genzyme) or control human IgG (Jackson ImmunoResearch) and 10% normal human complement (Quidel) in X-vivo 15 medium (BioWhittaker) supplemented with 10% FBS (Biochrom). ..

    Article Title: Impact of alemtuzumab treatment on the survival and function of human regulatory T cells in vitro
    Article Snippet: .. Complement-dependent cytolysis assay Enriched T cells were incubated for 1 hr at 37° with 10 μg/ml alemtuzumab or control human IgG (Jackson ImmunoResearch, West Grove, PA) and 10% (volume/volume) normal human complement (Quidel, San Diego, CA) at 2·5 x 10 6 cells/ml in complete AIM-V medium (AIM-V supplemented with 10% fetal bovine serum, 100 units/ml penicillin-streptomycin, 2 mM glutamine and 50 μ m β-mercaptoethanol; Life Technologies, Grand Island, NY). ..

    Article Title: Impact of alemtuzumab treatment on the survival and function of human regulatory T cells in vitro
    Article Snippet: .. Enriched T cells were incubated for 1 hr at 37° with 10 μg/ml alemtuzumab or control human IgG (Jackson ImmunoResearch, West Grove, PA) and 10% (volume/volume) normal human complement (Quidel, San Diego, CA) at 2·5 x 10 6 cells/ml in complete AIM-V medium (AIM-V supplemented with 10% fetal bovine serum, 100 units/ml penicillin-streptomycin, 2 mM glutamine and 50 μ m β-mercaptoethanol; Life Technologies, Grand Island, NY). ..

    Lysis:

    Article Title: An Fc variant with two mutations confers prolonged serum half-life and enhanced effector functions on IgG antibodies
    Article Snippet: .. The complement-induced lysis of CD20 + Raji cells was measured using a normal human complement (Quidel, San Diego, CA, USA) and a CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega). .. After adding 50 μl of Raji cells (1 × 10 4 cells) to each well of a sterile, clear, 96-well, V-bottom plate (Corning), 10 μl of rituximab-Fc variants, threefold serially diluted from 1 μg/ml, was added to each well.

    Cytotoxicity Assay:

    Article Title: An Fc variant with two mutations confers prolonged serum half-life and enhanced effector functions on IgG antibodies
    Article Snippet: .. The complement-induced lysis of CD20 + Raji cells was measured using a normal human complement (Quidel, San Diego, CA, USA) and a CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega). .. After adding 50 μl of Raji cells (1 × 10 4 cells) to each well of a sterile, clear, 96-well, V-bottom plate (Corning), 10 μl of rituximab-Fc variants, threefold serially diluted from 1 μg/ml, was added to each well.



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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    Quidel normal human serum nhs
    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human <t>complement.</t> Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.
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    Image Search Results


    ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Journal: Science Advances

    Article Title: Xenopus IgX informs engineering strategies of IgM and IgG hexamers

    doi: 10.1126/sciadv.aea3737

    Figure Lengend Snippet: ( A ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies were assessed using OCI-Ly10 cells in the presence of human complement. Data were plotted as the means ± SD. n = 3 biological replicates. Source data are provided in the Supplementary Materials. ( B ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Daudi cells. To facilitate the assay, the Daudi cells, and also the Raji cells in (C), were first cultured for several days in the RPMI-1640 medium supplemented with 10% heat-inactivated FBS. Data were plotted as the means ± SD. n = 3 biological replicates. ( C ) CDC activities of the engineered RTX-IgG and RTX-IgM antibodies toward the Raji cells. Data were plotted as the means ± SD. n = 3 biological replicates.

    Article Snippet: The RTX-Fcμ or RTX-Fcγ proteins were incubated with equal volumes of cell cultures (~20,000 cells) and 12% normal human serum complement (Quidel) sequentially and then transferred into a 96-microwell plate.

    Techniques: Cell Culture